- Title
- Efficacy of short-term cold storage prior to cryopreservation of spermatozoa in a threatened lizard
- Creator
- Campbell, Lachlan; Clulow, John; Howe, Belinda; Upton, Rose; Doody, Sean; Clulow, Simon
- Relation
- Reproduction, Fertility and Development Vol. 33, Issue 9, p. 555-561
- Publisher Link
- http://dx.doi.org/10.1071/RD20231
- Publisher
- C S I R O
- Resource Type
- journal article
- Date
- 2021
- Description
- Assisted reproductive technologies (ARTs) have a significant role to play in reptile conservation, yet are severely lacking. Previous attempts to cryopreserve spermatozoa in the threatened lizard Varanus panoptes achieved approximately 48% motile sperm post-thaw for samples frozen immediately after collection. However, the feasibility of extended cold storage before cryopreservation has not been tested. We held V. panoptes spermatozoa at either 258Cor 48C for 8 days, assessing sperm motility at days 1, 2, 4 and 8. Subsamples were cryopreserved on days 1 and 4 following the previously reported protocol for this species. Percentage motility decreased rapidly at 258C, but did not decrease significantly until 4 days after collection at 48C, with .30% motility maintained after 8 days. There was no significant difference in post-thaw motility or viability of samples cryopreserved after 1 or 4 days storage at 48C, yielding substantial results for both parameters (mean motility 23.8% and 28.1% and mean viability 50.1% and 57.5% after 1 and 4 days respectively). We demonstrate the capacity to extend sperm viability for up to 8 days in unfrozen samples and to produce acceptable post-thaw motility in samples frozen after 4 days of storage, contributing to the development of valuable ARTs for lizards and other reptiles.
- Subject
- assisted reproductive technologies; conservation; genome storage; reptile; squamate
- Identifier
- http://hdl.handle.net/${Handle}
- Identifier
- uon:49400
- Identifier
- ISSN:1031-3613
- Rights
- x
- Language
- eng
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